Page 37 - Hormel Report 2023

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Cilia expression on the surface of drug resistant lung cancer cells is increased relative to corresponding drug sensitive lung tumor cells. Cilia can be visualized by immunofluorescence staining for ARL13B (green) and γ-Tubulin (red), which are markers of cilia. DAPI (blue) stains the nucleus.
PUBLICATION
HIGHTLIGHTS:
• Phoenix, K. N., Yue, Z. C., Yue, L. X., Cronin, C. G., Liang, B. T., Hoeppner, L. H., & Claffey, K. P. (2022).
PLC beta 2 Promotes VEGF- Induced Vascular Permeability. Arteriosclerosis Thrombosis and Vascular Biology, 42(10), 1229-1241.
• Alam, S. K., Wang, L., Zhu, Z.,
& Hoeppner, L. H. (2023). IKKα promotes lung adenocarcinoma growth through ERK signaling activation via DARPP-32-mediated inhibition of PP1 activity.
npj Precision Oncology, 7(1), 16.
the way for the development of therapies to better combat therapy-refractory lung cancer progression (Oncogene, 2022). We are testing predictive biomarkers of acquired resistance and aiming to discover new ways to prevent EGFR inhibitor refractory lung cancer progression in work funded by the Lung Cancer Research Foundation and EGFR Resisters. We demonstrated that a protein called IKKα drives NSCLC growth through activation of oncogenic signaling via DARPP-32-mediated inhibition of protein phosphatase 1 activity (NPJ Precision Oncology, 2023). In newly conceived studies supported by a Paint the Town Pink pilot project grant, our group has shown that increased cilia formation in lung cancer cells is linked to the devel- opment of resistance to therapy (Figure 1). Cilia are microtubule-based sensory organelles that detect extracellular stimuli.
Vascular Permeability in Cardiovascular Disease, Lung Injury, and Cancer.
Vascular permeability triggered by inflammation or ischemia promotes edema, exacerbates disease progression, and impairs tissue recovery. We have used a variety of in vitro and in vivo models to understand the molecular basis of STAT3- regulated permeability (Disease Models & Mechanisms, 2021). We have also recently identified phospholipase C β2 as a key positive regulator of VEGF-induced vascular permeability (Arteriosclerosis, Thrombosis, & Vascular Biology, 2022).
ORCID iD: https://orcid.org/0000-0003-3948-4244