Page 47 - Hormel Report 2023

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protein 5 (BMP5). Lineage-depleted BMCs migrated towards HMGB1 protein and epidermal KCs in ex vivo invasion assays. Recipients receiving BMTs from DMBA-treated donors developed benign and malignant cutaneous lesions after TPA promotion alone. In summary, BMCs contribute to both initiation and promotion of cutaneous carcino- genesis. We conclude that a subset of squamous lesions long thought to be solely local in origin have a distinct systemic component. These findings may suggest new targets for diagnosis and surveillance of NMSCs.
ORCID iD: https://orcid.org/0000-0002-3858-1141
High risk solar UV treated skin, stained with CD3ε (red), K1 (pink), K13 (green), and DAPI (blue). Individual colors may be hard to see in the merged image. CD3ε is involved in T-cell activation. The Keratins indicate different levels of differentiation in epithelial cells. DAPI is a general nuclear stain.
High risk solar UV exposed skin stained with PCNA (pink) and DAPI (blue). PCNA (proliferating cell nuclear antigen) is expressed in cells that are actively dividing. DAPI is a general nuclear stain.
PUBLICATION HIGHLIGHTS:
• Wang, Q., Morris, R. J., Bode, A. M., & Zhang, T. (2022). Prostaglandin Pathways: Opportunities for Cancer Prevention and Therapy. Cancer Research, 82(6), 949-965.
• Holtorf, S. M., Boyle, J., & Morris, R. (2023). Evidence for EpCAM and Cytokeratin Expressing Epithelial Cells in Normal Human and Murine Blood and Bone Marrow. Journal of Visualized Experiments, 2023(194).
High risk skin tumor using a two-step carcinogenesis model. First, the tumor was initiated using carcinogen-exposed bone marrow, followed by a bone marrow transplant. Second, the tumor was promoted with a skin irritant. The carcinogen-exposed bone marrow cells (indicated in red) traveled to the skin where the irritant was applied and started to express a marker for skin, Pankeratin (indicated in green). DAPI is a general nuclear stain.