Page 59 - Hormel Report 2023

Dpb3 deletion mutant (dpb3Δ), and the double mutant (mcm2-3A/ dpb3Δ), likely due to the elevated free histone level. These results confirmed that proper transfer of parental histones onto the leading and lagging strands during DNA replication is critical to maintain normal chromatin structure. Moreover, using the eSPAN approach, we recently found that Iron-sulfur (Fe/S) clusters (ISCs) pathway is also involving in the regulation of parental histone transfer onto the DNA lagging strands. ISCs are known as redox-active protein cofactors essential to redox catalysis in nitrogen fixation, oxidative phosphorylation, mitochondrial respiratory and DNA metabolism. Our genetic analyses in yeast indicated that mutations of the ISCs pathway led to a frequent loss of silence at the mating type locus that is normally silenced. The results revealed a new mechanism of ISCs that impacts epigenetic inheritance through regulating parental histone transfer.
ORCID iD: https://orcid.org/0000-0003-1885-7224
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PUBLICATION HIGHLIGHTS:
• Serra-Cardona, A., Duan, S. F., Yu, C. H., & Zhang, Z. G. (2022). H3K4me3 recognition by the COMPASS complex facilitates the restoration of this histone mark following DNA replication. Science Advances, 8(18), 16.
• Tian, C. C., Zhou, J. Q., Li, X. R., Gao, Y., Wen, Q., Kang, X., Wang, N., Yao, Y., Jiang, J. H., Song, G. B., Zhang, T. J., Hu, S. L., Liao, J. Y., Yu, C. H., Wang, Z. Q., Liu, X. Y., Pei, X. H., Chan, K. M., Liu, Z. C., & Gan, H. Y. (2023). Impaired histone inheritance promotes tumor progression. Nature Communications, 14(1), 17.
• Wang, Z. Q., Yan, H. H., Boysen, J. C., Secreto, C. R., Tschumper, R. C., Ali, D., Guo, Q. Q., Zhong, J., Zhou, J. Q., Gan, H. Y., Yu, C. H., Jelinek, D. F., Slager, S. L., Parikh, S. A., Braggio, E., & Kay, N. E. (2022). B cell receptor signaling drives APOBEC3 expression via direct enhancer regulation in chronic lymphocytic leukemia B cells. Blood Cancer Journal, 12(7), 11.
CURRENT RESEARCH PROJECTS:
1) Regulation mechanism of parental histone assembly in yeast.
2) Genetic interaction of parental and new histone chaperones
in yeast.
3) New approaches to study DNA replication coupled process.