Page 7 - IBP In Focus Newsletter 2020

t IBP AT A GLANCE Pilar Ariza Guzman, DVM, MS ariza001@umn.edu / 612-624-0974
Core Contact:
Physiology Core
The Physiology Core (formerly known as IBP Phenotyping Core) is an Internal Service Organization (ISO) created to offer University of Minnesota faculty highly specialized equipment, surgical and technical expertise and data analysis capability in the area of metabolic and cardiovascular phenotyping and stress physiology (https://www.researchservices.umn.edu/ services-name/physiology-core). Alessandro Bartolomucci, Ph.D., is the Core Director, Pilar Ariza-Guzman, D.V.M., M.S., is Core Manager, and Maria Razzoli, Ph.D., is the Metabolism and Behavior Specialist.
Major services include:
• Whole animal energy expenditure: Oxymax/CLAMS Lab Animal Monitoring System (Columbus Instruments,
Columbus, OH).
• Body composition and organ fat and fat-free composition (Echo-MRI 3-in-1, Echo Medical Systems LLC, Houston, TX).
• Automated Food Consumption and meal pattern analysis (BioDaq, Reseach Diets, Inc.).
• Cardiovascular functions in freely moving rodents using Data Science’s radiotelemetry system (Data
ScienceInternational, St Paul, MN). New to the Core is the acquisition in collaboration with the Lillehai Heart Institute
of a 16 channels Ponemah system to allows automated ECG analysis.
• Non Invasive Blood Pressure Measurement.
• Specialized surgery and echocardiography analysis in mice and rats.
Economic impact of the core:
Approximately 70% of the billings were from sponsored accounts. For all those grants (intramural and extramural), independently from the $ spent in the core, there is no alternative to the service offered by the Physiology Core for performing the proposed experiments.
The vast majority of these grants were from NIH, others included: ADA, AHA, etc. The total annual direct cost awarded to the grants used to pay Core service in fiscal year 2020 is over $3.9M, and the total indirect costs is over $1.7M. This is an underestimation of the economic impact of the core because it is difficult to provide similar figures for intramural grants (some of which are competitive grants such as the MN Partnership, Wallin Neuroscience Discovery Fund, etc).
Scientific impact of the core:
Since its inception in 2015, the Core has served over 70 PIs affiliated with more than 15 different departments, spanning four different schools at the University of Minnesota. In FY20 the Core supported 30 grant proposals (NIH, ADA, Foundations, etc.) that included one or more IBP Phenotyping Core service.
A few examples of papers published in 2019-2020 which were supported by the Core is listed below:
Najt CP, Khan SA, Heden TD, Witthuhn BA, Perez M, Mead LE, Franklin MP, Karanja KK, Graham MJ, Mashek MT, Bernlohr DA, Parker L, Chow LS, Mashek DG. Lipid droplet-derived monounsaturated fatty acids traffic via PLIN5 to allosterically activate SIRT1. Molecular Cell 2020;77(4):810-824.
Deis JA, Guo H, Wu Y, Liu C, Bernlohr DA, Chen X. Adipose Lipocalin 2 overexpression protects against age-related decline in thermogenic function of adipose tissue and metabolic deterioration. Mol Metab. 2019 Jun;24:18-29.
Zhang Z, Huang Z, Ong B, Sahu C, Zeng H, Ruan HB. Bone marrow adipose tissue-derived stem cell factor mediates metabolic regulation of hematopoiesis. Haematologica. 2019. doi: 10.3324/haematol.2018.205856.
Razzoli M, Lindsay A, Law ML, Chamberlain CM, Southern WM, Berg M, Osborn J, Engeland WC, Metzger JM, Ervasti JM, Bartolomucci A. Social stress is lethal in the mdx model of Duchenne Muscular Dystrophy. EBioMedicine, 2020;55;102700.
The main Core facility is located in the Cardio-Cancer Research Building (CCRB), while select services are also available on the 1st floor of Jackson Hall.
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